Prevalence of Genes Encoding Resistance to Aminoglycosides and Virulence Factors Among Intestinal Vancomycin-Resistant Enterococci

Background: Vancomycin-resistant enterococci (VRE) are recognized as nosocomial pathogens with increased importance in recent years. These bacteria frequently isolated from patients admitted to intensive care units (ICUs). Enterococcal pathogenicity is enhanced by different antibiotic resistance and virulence determinants. Objectives: The present study aimed assess the prevalence of genes encoding antibiotics factors intestinal VRE isolates ICU patients. Methods: In this study, 23 VREs were investigated. Minimum inhibitory concentrations (MICs) nine antimicrobial agents examined using E-test. Genes vancomycin (vanABCDMN), aminoglycoside-modifying enzymes (aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, ant(3')-Ia, ant(4')-Ia, ant(6')-Ia), together for various factor (ace/acm, asa1, cylA, efaA, esp, gelE hyl), detected multiplex PCR. Results: species distribution tested was follows: Nine Enterococcus casseliflavus, seven E. gallinarum, faecium. vanA gene found all faecium, six which classical VanA phenotype observed. (vanC) associated presence vanC1 gallinarum vanC2 casseliflavus isolates. aac(6')-Ie-aph(2")-Ia high-level gentamicin (HLGR) studied VRE. All faecium positive acm while combination esp or hyl 2 vanC enterococci. Conclusions: According findings, there a correlation between genotype glycopeptide HLGR more prevalent because aac(6')-Ie-aph(2")-Ia. higher determinants confirmed compared vanC-carrying